Connection property has not been initialized while running application from IIS

I have one intranet based application.I have set windows authentication for it in Web.config as well as from 开发者_如何学JAVAIIS settings.

While running my application through the source code, it works fine but while running from directly IIS then its giving me:

"Connection property has not been initialized"

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Probably the problem is IIS websites runs with different account - usually it's NETWORK SERVICE